METHODS

Islet Sheet Medical Useful Methods

Overview of Methods

Edited by Randy Dorian, CSO

March 20, 2000


How to make "Randy's Best" microcapsules

Randy explains how to make small, bioinvisible alginate capsules that cover 100% of the islets.


"Bioinvisible" high mannuronate alginate

"Bioinvisible" high mannuronate alginate 1 U.S. Patent 5,429,821 (July 4, 1995)

"Bioinvisible" high mannuronate alginate 2 (March 31, 1999)

"Bioinvisible" high guluronate alginate

"Bioinvisible" high guluronate alginate 1 U.S. Patent 5,643,594 (July 1, 1997)

"Bioinvisible" high guluronate alginate 2 (March 31, 1999)


Introduction

The technology for making bio-artificial pancreases is improving rapidly. Before working on methods to make thin sheets, we worked for years on microencapsulation. We developed many useful methods. We are happy to share our knowledge, both on this web site, and in real time: e-mail or call us with questions.

Islet Sheet Medical provides these "Useful Methods" as an aid to researchers in the islet encapsulation field. We encourage anyone who has alternative methods or other useful tips to submit them to us for posting to this page. (Final judgment for posting these methods will be made by ISM.) We will not publish any bad methods.

Please feel free to contact us if you need further technical details on posted methods or have any questions about these or other methods. Our hope is to provide a resource of valuable tools which will help our colleagues to progress more efficiently toward our common goal of curing juvenile diabetes.

Initially we are posting methods for making "bioinvisible" alginate. Soon we hope to add methods for making thin capsules, including the electrostatic, spinning disk, air knife, and "halo" methods.

Citation

These methods are a web resource provided by Islet Sheet Medical to encourage technological advance. We would appreciate a citation when one of these methods is used. Each method page will be left on our server without change (except for typographical-type errors and clarifications). Improvements will be posted on a new page with a new, higher number.

Sample citation:

Method for purification of high mannuronate alginate:

Dorian, Randy, Ed., Islet Sheet Medical Useful Methods, http://www.isletmedical.com/meth0102 (March 31, 1999).


Useful Methods

"Bioinvisible" high mannuronate alginate

"Bioinvisible" high mannuronate alginate 1 U.S. Patent 5,429,821 (July 4, 1995)

"Bioinvisible" high mannuronate alginate 2 (March 31, 1999)

"Bioinvisible" high guluronate alginate

"Bioinvisible" high guluronate alginate 1 U.S. Patent 5,643,594 (July 1, 1997)

"Bioinvisible" high guluronate alginate 2 (March 31, 1999)